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. 2016 Dec 27;8(4):6589–6607. doi: 10.18632/oncotarget.14244

Figure 3. Effect of GAS5 knockdown on human neuroblastoma cells.

Figure 3

A. Loss of GAS5 in IMR-32 cells decreased apoptosis rate at both 24 and 48 hr time points. B. Loss of GAS5 in IMR-32 cells decreased cell proliferation rate at both 24 and 48 hr time points. C and D. Knockdown of GAS5 in IMR-32 cells increased the content of G0/G1 cells and decreased the content of G2/M phase cells, inducing cell cycle arrest, as measured by propidium iodide staining. E. Loss of GAS5 in SK-N-AS cells caused no significant changes in apoptosis rate at either 24 or 48 hr time points. F. Loss of GAS5 in SK-N-AS cells had no detectable effect on cell proliferation rate at both 24 and 48 hr time points. G and H. Knockdown of GAS5 in SK-N-AS cells yielded no significant changes in either G0/G1 or G2/M phase cells as measured by propidium iodide staining. All experiments were performed in triplicate. * p < 0.05, Student's t-test.