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. 2016 Dec 27;8(4):6589–6607. doi: 10.18632/oncotarget.14244

Figure 4. Differential modulations of cellular functions by GAS5 splice variants, FL and C2, in IMR-32 cells.

Figure 4

A. Measurement of apoptosis rate in IMR-32 cells knocked-down for GAS5 and complemented with individual variants FL or C2. Both variants can compensate for a decrease in apoptosis due to global loss of GAS5. However, the C2 variant increases apoptosis rate slightly more than FL. B. Measurement of cell viability in IMR-32 cells knocked-down for GAS5 and complemented with individual variants FL or C2. Both variants can compensate for viability loss due to global decrease in GAS5. However, the FL variant increases proliferation considerably more than C2. C-E. Measurement of cell cycle populations after knockdown of GAS5 followed by complementation with C) the FL splice variant or D) the C2 splice variant in IMR-32 cells. Complementation of the FL variant appears to rescue cell cycle arrest induced by loss of GAS5, whereas the C2 variant does not appear to do so. All experiments were performed in triplicate. * and ** p < 0.05, Student's t-test.