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Schematic representation of the BlaI cassette. The blaI gene is under the control of its own promoter (PblaI). Spcr indicates the spectinomycin resistance gene from pIC333. Rectangles represent the operator DNA sequence recognized by the BlaI repressor. repfront and repback contain direct repeat unit sequences (138 bp) used for the eviction of the BlaI cassette. For details see the text.
