TABLE 3.
Synthetic primers for PCR amplification
| Name | Length (bp) | Sequence (5′ → 3′) |
|---|---|---|
| BlaIbam+ | 30 | ATATGGATCCATCAAAATCGTCTCCCTCCG |
| promblaPBglII | 32 | ATCCAGATCTTCCCTCCGTTCATTTGTCCCCG |
| lysABamHI | 34 | ATGGATCCACACGGCACAAGCAGACAAAATCAAC |
| lysAend | 25 | CATTGATTTCTTCGTATCTATCTGG |
| repGFPbackup | 33 | GATATCGGATCCTTTTACCAGACAACCATTACC |
| repGFPbackdo | 27 | AGATCTATGACCATGATTACGCCAAGC |
| repGFPfrup | 30 | GTCGACTCCTTTTACCAGACAACCATTACC |
| repGFPfrdo | 33 | GATATCTCTAGATTTGTATAGTTCATCCATGCC |
| spoVAFBglI | 31 | AGATCTTGCGATTATGAATTGGTAGGCTGCC |
| spoVAFMluI | 27 | ACGCGTATGCCGGACCACAAGGAAGAG |
| pBlalBglII | 30 | CAGATCTAAGTGATGGAATTAAAAATGCAG |
| stopBlaIBamHI | 31 | CGGATCCTCATTCCTTCTTTCTGTTCTTATG |
| Spc+ | 31 | GGAAGTTCAATACTTGGAGTATATCTATTTG |
| Spc− | 21 | CTTATCATCACACTCTCCCCG |
| BlaP+ | 33 | CGCTTCGATATAGTGACAATGCGGCACAGAATC |
| BlaP− | 31 | GCCGTTCATGTTTAAGGCTTTCATTACCACC |
| BlaINdeI | 30 | ATACATATGAAAAAAATACCTCAAATCTCTG |
| BlaIEcoRI | 37 | ATAGAATTCATTTCATTCCTTCTTTCTGTTCTTATG |
| kanaup | 22 | ATGGCTAAAATGAGAATATCAC |
| kanadown | 21 | CTAAAACAATTCATCCAGTAA |
| amyEfront | 25 | TTTATTGCTGTTTCATTTGGTTCTG |
| amyEback | 24 | GATGGTGTATGTTTTGCCAAATTG |