Figure 3. Cordycepin inactivates mTOR and leads to autophagy in NSCLC cells.

A and D. H460, H1299 and H157 cell lines were treated with 0, 50, 100 and 200 μM cordycepin for 12 h. B. H460 and H1299 cells were conducted with Atg5 siRNA and treated with 100 μM cordycepin for 12 h. C. H1299 cells were pretreated with10 μM LY294002 or 20 μM CQ for 1h and then exposed to 100 μM cordycepin for another 12 h. Then whole-cell protein lysates were harvested and prepared for western blot analysis.