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. 2016 Dec 27;8(4):6691–6699. doi: 10.18632/oncotarget.14262

Figure 4. Cordycepin gives rise to pro-apoptotic autophagy in NSCLC cells.

Figure 4

H1299 and H157 cell lines were seeded in six-well plates and conducted with Atg5 siRNA transfection on the next day. After Forty-eight hours transfection, cells were exposed to 200 μM cordycepin for 12 h. Subsequently, cells were harvested and prepared for western blot analysis A. or subjected to flow cytometry analysis after 24 h treatment B. H1299 and H460 cell lines were seeded in six-well plates and pretreated with 10 μM LY294002 for 1h on the next day, then cells were exposed to 200 μM cordycepin for 12 h. Subsequently, cells were harvested and prepared for western blot analysis C. or subjected to flow cytometry analysis after 24 h treatment D. Data represented mean ± SEM from three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001)