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. 2016 Dec 27;8(4):6796–6808. doi: 10.18632/oncotarget.14296

Figure 2. AR-Q784* mutant is deficient for androgen stimulated transcription activity.

Figure 2

A, B, C. Dual-luciferase reporter assays were carried out in PC-3 cells transfected with empty vector, AR-FL, AR-V7, and AR-Q784* using various androgen-regulated reporters driven by (A) ARE4, (B) MMTV promoter, or (C) Probasin promoter, and treated with vehicle or 10nM DHT (24h). Reporter activity was normalized to the cotransfected CMV-Renilla-Luc. D. Endogenous FKBP5 mRNA expression was examined by qRT-PCR in PC-3 cells transfected with empty vector, AR-FL, or AR-Q784*, and then treated with 10nM DHT (24h). E, F, G. Dual-luciferase reporter assays were performed in COS-7 cells transfected with empty vector, AR-FL, or AR-Q784*, and treated with the indicated small molecules using reporters driven by (E) ARE4, (F) Probasin promoter, or (G) PSA enhancer.