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A. Immunofluorescence staining for AR (N-terminal) (green) in COS-7 cells transfected with AR-FL or AR-Q784* and treated with 10nM DHT for 24h. Nuclear compartment was determined by DAPI staining (blue). B. ChIP-qPCR for measuring AR binding (N-terminal) at an FKBP5 enhancer in PC-3 cells transfected with empty vector, AR-FL, AR-V7, or AR-Q784*, and treated with vehicle or 10nM DHT (4h).
