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. 2016 Dec 27;8(4):6796–6808. doi: 10.18632/oncotarget.14296

Figure 5. AR-Q784* heterodimerizes with AR-FL and enhances its transcriptional activity.

Figure 5

A, B. Dual-luciferase reporter assays were carried out in PC-3 cells transfected with empty vector, AR-FL, AR-Q784*, or AR-FL+AR-Q784* using androgen-regulated reporters driven by (A) ARE4 or (B) PSA enhancer, and treated with vehicle or 10nM DHT (24h). C. Immunoprecipitation for HA in COS-7 cells cotransfected with HA-tagged AR-FL plus FLAG-tagged AR-FL or AR-Q784*, and treated with vehicle or 10nM DHT (24h), followed by immunoblotting for FLAG or HA. D. COS-7 cells transfected with empty vector, AR-FL, AR-Q784*, or AR-FL plus AR-Q784*, and treated with vehicle or DHT were fractionated into nuclear and cytoplasmic extracts, followed by immunoblotting for AR (N-terminal). GAPDH is a loading control for whole cell extracts and cytoplasmic fraction. Lamin B1 is a loading control for nuclear fraction.