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A, B. ChIP-qPCR for measuring (A) AR-FL binding (using antibody against C-terminal region) or (B) p300 binding at an FKBP5 enhancer in LNCaP-AR-Q784* cells pretreated with vehicle or doxycycline and then stimulated by DHT (0, 0.1 or 1 nM) for 4h.
