Table 3. miRNA expression in the frozen tissue and pathological characteristics compared with and without recurrence.
| Patients with recurrencen=14, (%) | Patients without recurrencen=66, (%) | Chi-square | Multivariate analysis for recurrence | 3 yearRFS rate (%) | log-rank | Multivariate analysis for RFS | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P value | Odds ratio | 95% C.I. | P value | P value | Hazard ratio | 95% C.I. | P value | ||||||
| let-7a | 0.045 | 0.352 | 0.040 | 0.367 | |||||||||
| High (n=18) | 6 | (42.9) | 12 | (18.2) | 66.7 | ||||||||
| Low (n=62) | 8 | (57.1) | 54 | (81.8) | 90.3 | ||||||||
| let-7d | 0.006 | 0.086 | 0.007 | 0.103 | |||||||||
| High (n=48) | 13 | (92.9) | 35 | (53.0) | 79.2 | ||||||||
| Low (n=32) | 1 | (7.1) | 31 | (47.0) | 96.9 | ||||||||
| let-7e | 0.003 | 0.121 | 0.002 | 0.167 | |||||||||
| High (n=13) | 6 | (42.9) | 7 | (10.6) | 61.5 | ||||||||
| Low (n=67) | 8 | (57.1) | 59 | (89.4) | 91.0 | ||||||||
| miR-23c | 0.008 | 0.086 | 0.009 | 0.135 | |||||||||
| High (n=27) | 9 | (64.3) | 18 | (27.3) | 74.1 | ||||||||
| Low (n=53) | 5 | (35.7) | 48 | (72.7) | 92.5 | ||||||||
| miR-26b | 0.001 | 0.171 | 0.001 | 0.213 | |||||||||
| High (n=11) | 5 | (35.7) | 6 | (9.1) | 55.6 | ||||||||
| Low (n=69) | 9 | (64.3) | 60 | (90.9) | 90.1 | ||||||||
| miR-128a | 0.001 | 0.264 | 0.006 | 0.216 | |||||||||
| High (n=11) | 5 | (35.7) | 6 | (9.1) | 63.4 | ||||||||
| Low (n=69) | 9 | (64.3) | 60 | (90.9) | 88.4 | ||||||||
| miR-148b | 0.079 | 0.558 | 0.082 | 0.490 | |||||||||
| High (n=46) | 11 | (78.6) | 35 | (53.0) | 80.4 | ||||||||
| Low (n=34) | 3 | (21.4) | 31 | (47.0) | 94.1 | ||||||||
| miR-151-5p | 0.012 | 0.883 | 0.118 | 0.780 | |||||||||
| High (n=31) | 8 | (57.1) | 23 | (34.9) | 77.4 | ||||||||
| Low (n=49) | 6 | (42.9) | 43 | (65.1) | 89.8 | ||||||||
| miR-181c | < 0.001 | 9.43 | 2.57 – 34.48 | 0.001 | < 0.001 | 6.62 | 2.08 – 21.28 | 0.001 | |||||
| High (n=24) | 10 | (71.4) | 14 | (21.2) | 70.8 | ||||||||
| Low (n=56) | 4 | (28.6) | 52 | (78.8) | 92.9 | ||||||||
| miR-361 | 0.088 | 0.645 | 0.094 | 0.579 | |||||||||
| High (n=35) | 9 | (64.3) | 26 | (39.4) | 80.0 | ||||||||
| Low (n=45) | 5 | (35.7) | 40 | (60.6) | 91.1 | ||||||||
| Tumor depth | 0.048 | 0.222 | 0.094 | 0.3191 | |||||||||
| T4 (n=14) | 5 | (35.7) | 9 | (13.6) | 75.0 | ||||||||
| T3 (n=66) | 9 | (64.3) | 57 | (86.4) | 88.2 | ||||||||
| Histology | 0.022 | 0.125 | 0.002 | 0.105 | |||||||||
| Muc (n=3) | 2 | (14.3) | 1 | (1.5) | 33.3 | ||||||||
| W/D and M/D (n=77) | 12 | (85.7) | 65 | (98.5) | 88.3 | ||||||||
C.I.: confidence interval, W/D: well-differentiated adenocarcinoma, M/D: moderately differentiated adenocarcinoma, Muc: mucinous carcinoma, High: higher expression of miRNA than the threshold, Low: equal or lower expression of miRNA than the threshold. The differences were analyzed by chi-square test, log-rank test or Multivariate logistic regression analysis. P < 0.05 denotes statistically significant difference.