Table 5. . Matrix effect of enalapril and enalaprilat calculated from six different source of human plasma.
| Analytes |
Matrix effect (ME %)† |
Mean | SD | % CV | |||||
|---|---|---|---|---|---|---|---|---|---|
| SET 1‡ | SET 2 | SET 3 | SET 4 | SET 5 | SET 6 | ||||
|
Enalapril | |||||||||
| LQC (3 ng/ml) |
95.8510 |
104.6398 |
106.0474 |
92.9591 |
109.0460 |
94.7069 |
100.5417 |
6.8260 |
6.7893 |
| MQC (200 ng/ml) |
113.3058 |
105.8869 |
90.9534 |
112.3145 |
91.5637 |
96.4744 |
101.7498 |
10.1036 |
9.9299 |
| HQC (400 ng/ml) |
103.3769 |
92.1597 |
99.1176 |
105.8241 |
94.9351 |
97.4031 |
98.8028 |
5.1258 |
5.1879 |
|
Enalaprilat | |||||||||
| LQC (3 ng/ml) |
106.9987 |
96.3672 |
110.2372 |
94.5207 |
95.3574 |
105.6815 |
101.5271 |
6.8825 |
6.7789 |
| MQC (200 ng/ml) |
94.9127 |
109.5726 |
101.5992 |
98.4407 |
105.2650 |
97.6180 |
101.2347 |
5.4176 |
5.3515 |
| HQC (400 ng/ml) | 95.3538 | 113.0894 | 91.0844 | 106.6942 | 90.7440 | 95.3352 | 98.7168 | 9.1079 | 9.2263 |
†ME% expressed as the ratio of the mean peak area of an analyte spiked post extraction to the mean peak area of the same analyte standards multiplied by 100. A value of >100% indicates ionization enhancement, and a value of <100% indicates ionization suppression.
‡SET 1–6 represent the ME% from six different source of human plasma.
HQC: High quality control; LQC: Low quality control; ME: Matrix effect; MQC: Medium quality control.