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Schematic overview of the dUC-ODG protocol to isolate EVs from cancer-associated adipose tissue-derived conditioned medium (CMCAAT). Approximately 21 g of CAAT was ex vivo cultivated in control medium. CMCAAT was harvested, centrifuged and used for further isolation by a combination of differential ultracentrifugation followed by Optiprep density gradient centrifugation.
