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. 2017 Feb 1;11(2):196–204. doi: 10.1080/19336918.2017.1279784

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Figure 4. — Stimulation of CREB transcription factor phosphorylation and sphere formation by EVs. EVs were isolated from the conditioned medium of cancer-associated adipose tissue by the dUC-ODG protocol. (A) Western blot analysis of phospho-CREB and total CREB from lysates of ZR75.1 cells under control conditions or treated by 1×10⁸ EVs. (B) The number of aggregates formed by MCF7 single cells seeded in ultra-low attachment plates under control conditions or treated by 5×10⁹ EVs for 24h. Each experiment was performed in quadruplicate.