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13C NMR spectra of the C-2 of alanine and illustration of the signal fine structure composition. s, singlet peak of [2-13C]alanine (no neighboring labels); d−1, 13C in the preceding position ([1,2-13C2]alanine) produces a doublet peak, split by scalar coupling; d+1, 13C in the following position ([2,3-13C2]alanine) yields another doublet split with a different coupling constant; dd, “doublet of doublet” signal of [13C3]alanine.
