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. Author manuscript; available in PMC: 2018 Jan 12.
Published in final edited form as: Cell. 2017 Jan 5;168(1-2):59–72.e13. doi: 10.1016/j.cell.2016.12.011

Figure 6. IRAK inhibition displaces MLL chimera and SEC occupancy at a subset of MLL chimera and SEC target genes.

Figure 6

(A) IRAK inhibitors decrease MLL-AFF1 and SEC occupancy at the LGALS1, GNA15 and LMO2 genes in SEM cells. Genome browser views of MLL-AFF1 (AFF1-CT) and SEC component AFF4 occupancy at the LGALS1, GNA15 and LMO2 genes are shown. Red boxes indicate the promoter-proximal regions with decreased MLL-AFF1 and AFF4 occupancy.

(B–C) We identified 1311 promoter regions (± 3kb of the TSS) in which MLL-AFF1 occupancy was altered by IRAK inhibitors. These regions are plotted as heatmaps (B) and metagene plots, and the Wilcoxon signed-rank test was used to show that MLL-AFF1 occupancy is significantly decreased after IRAK inhibition (C).

(D–E) Heatmap, metagene and statistical analysis of AFF4 occupancy at the 1311 promoter regions.

(F) RNA-seq genome browser track examples indicating that AFF4 knockdown reduces the expression of the LGALS1, GNA15 and LMO2 genes in SEM cells.

See also Figures S6.

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