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. Author manuscript; available in PMC: 2017 Mar 15.
Published in final edited form as: Nat Immunol. 2008 Sep 14;9(10):1189–1197. doi: 10.1038/ni.1654

Figure 1. Macrophages undergoing apoptosis form a surface matrix containing cross-linked annexin-1 and PAI2.

Figure 1

(a) Immunoblot for annexin-1 or actin on lysates of human macrophages infected with Mtb strain H37Ra undergoing apoptosis and strain H37Rv undergoing necrosis (MOI 5 and 2) for 48 h. The arrow indicates high molecular weight annexin-1, which is decreased in necrotic macrophages. (b) Immunoblot for annexin-1 on lysates of H37Ra-infected (MOI 10) and uninfected macrophages surface-extracted with 2 mM EDTA in PBS. The EDTA wash and the lysed cells were immunoblotted for annexin-1. High molecular weight annexin-1 (arrow). (c,d) Immunoassays on RAW 264.7 cells treated with 5 μM etoposide (etop) or control medium for 48 h and then extracted with EDTA and immunoblotted for annexin-1 and PAI2 (c) or immunoprecipitated with annexin-1 antibody and immunoblotted for PAI2, or annexin-1 as loading control (d). (e) Immunoblot for annexin-1 or actin on lysates of RAW 264.7 cells treated with etoposide or control medium in the presence or absence of cystamine. One experiment of three is shown.