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. Author manuscript; available in PMC: 2017 Mar 15.
Published in final edited form as: Nat Immunol. 2008 Sep 14;9(10):1189–1197. doi: 10.1038/ni.1654

Figure 4. Effect of virulent H37Rv on PAI2 mRNA accumulation.

Figure 4

(a) RT-PCR for PAI2 and actin mRNAs in macrophages (5 ×105 / plate) cultured with or without LPS (10 ng/ml) and infected at the MOI 10 for 24 h with virulent H37Rv. Ratio of expression refers to the n-fold-difference in PAI2 mRNA concentrations normalized to actin mRNA. (*P = 0.05, ** P = 0.01, n = 4). (b) Virulent H37Rv in contrast to avirulent H37Ra induce production of significant amounts of LXA4. Macrophages were cultured with H37Ra or H37Rv at the MOI indicated and LXA4 was measured in the supernatants after 12 h. (*P = 0.001, n = 3). (c) RT-PCR for PAI2 and actin mRNAs in macrophages infected with or without H37Ra (MOI 10, 24 h) in presence and absence of LXA4 at the concentrations indicated. Ratio of expression refers to the n-fold-difference in PAI2 mRNA concentrations normalized to actin mRNA (*P = 0.001, ** P = 0.03, n = 3).