FIG. 2.
Mass isotopomer distributions of the carbon skeletons of glucose-6-phosphate (A) and lysine (B) during cultivation of C. glutamicum ATCC 21526 on [1-13CFru]sucrose, [1-13CGlc]sucrose, and [13C6Fru]sucrose, respectively. The data were calculated from GC-MS measurements of the m/z 361 ion cluster of trimethylsilyl-derivatized trehalose, corresponding to the carbon skeleton of glucose-6-phosphate, and of the m/z 431 ion cluster of t-butyl-trimethylsilyl-derivatized lysine by correcting for natural isotopes as described by van Winden et al. (21).