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. Author manuscript; available in PMC: 2017 Mar 15.
Published in final edited form as: DNA Repair (Amst). 2016 Mar 2;40:67–76. doi: 10.1016/j.dnarep.2016.02.002

Fig. 2. The important role of HERC2 and RNF8 in TLS past abasic sites during Ig Vλ hypermutation.

Fig. 2

(A) Clones over-expressing AID were expanded for two weeks before isolating genomic DNA and sequencing the Vλ segment. Charts displaying the frequency of each type of mutation. Segment size indicates the proportion of cells in which the number of mutations stated outside the chart had occurred. The total number of sequences analyzed is shown in the centre of the chart. PM = Point mutation/non-templated single base substitution; AMB = Ambiguous; GC = Gene conversion. (B) The rate of point mutation/non-templated single base substitution (PM) events in wild-type cells were set to 1.00 and all values were normalized to the value of wild-type cells and are indicated with standard error. (C) Pattern of point mutation in the indicated cell lines. Tables showing the pattern of mutation in each line, given a percentage of mutations observed.