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. Author manuscript; available in PMC: 2017 Mar 15.
Published in final edited form as: DNA Repair (Amst). 2016 Mar 2;40:67–76. doi: 10.1016/j.dnarep.2016.02.002

Fig. 3. Neither HERC2 nor RNF8 is required for the mono-ubiquitination of PCNA in UV-irradiated cells.

Fig. 3

(A) Analysis of ubiquitination of PCNA. Cells were irradiated with 30 J/m2 UV and then incubated for 1 h. Protein extracts were prepared in native conditions for mono-ubiquitination, and analyzed by gel-electrophoresis followed by western blotting using an anti-PCNA antibody. (B) Quantification of ubiquitinated PCNA by immunoblotting. The values for ubiquitinated PCNA were calculated as described in the Materials and Methods. Error bars indicate standard deviation (SD). (C) Analysis of ubiquitination of PCNA in RAD18-/- and RAD18-/-/RAD18-/- cells as in fig. 3A.