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. Author manuscript; available in PMC: 2017 Mar 15.
Published in final edited form as: DNA Repair (Amst). 2016 Mar 2;40:67–76. doi: 10.1016/j.dnarep.2016.02.002

Fig. 5. HERC2 and RNF8 are dispensable to maintain the velocity of unperturbed DNA synthesis.

Fig. 5

(A) Schematic of treatment with UV and pulse labeling with IdU and CldU are shown. Representative image showing stained DNA fibres. (B) Replication fork lengths were obtained by converting the IdU track sizes in µM to kb and analyzed in the indicated cell types. IdU track lengths are calculated by dividing the track lengths by the labeling time and depicted above the track lengths. More than 100 forks were calculated in each cell types. The P-values were calculated by Student’s t-test (C) Indicated cell lines were left untreated and DNA fibres were analyzed for inter-origin distances (IODs). IODs were measured as the distance between two adjacent initiation sites during IdU pulse, and average values are indicated in kb. Over 50 fibers were analyzed in measurement of IODs. The P-values were calculated by Student’s t-test. (D) CldU track lengths were analyzed in the indicated cell types as previous. The P-values for Student’s t-test were *p<0.001. (E) Indicated cell lines were treated with UV and DNA fibres were analyzed for inter-origin distances (IODs) as previous.