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. 2004 Dec;70(12):7436–7444. doi: 10.1128/AEM.70.12.7436-7444.2004

FIG. 2.

FIG. 2.

Agarose gel electrophoresis showing the results of multiplex PCR amplification of three Vibrio spp. (A) V. vulnificus: lane 1, DNA size standard (Clone-sizer; Norgen Biotek); lane 2, multiplex PCR of both vvh and viuB targets; lane 3, 205-bp vvh amplicon; lane 4, 504-bp viuB amplicon. (B) V. parahaemolyticus: lane 1, DNA size standard; lane 2, 450-bp tlh amplicon; lane 3, 269-bp tdh amplicon; lane 4, 500-bp trh amplicon; lane 5, 369-bp ORF8 amplicon; lane 6, multiplex PCR amplification of all four targeted gene segments. (C) V. cholerae: lane 1, DNA size standard; lane 2, 779-bp toxR amplicon; lane 3, 869-bp ompU amplicon; lane 4, 862-bp tcpI amplicon; lane 5, 738-bp hlyA amplicon; lane 6, multiplex PCR amplification of all four targeted gene segments.