TABLE 1.
Primer | Sequence (5′-3′) |
---|---|
MGL−148 | TTT TCC CCA CGC CCC GTG |
MGL+148 | GCA TGA CGA AAA GTG ACA |
MGL-F | ATG AGC ATC ACC CAG AAC |
MGL-R | TCA TAC CGT TGC TAC AGG |
FragMGL-F | TCG ACC TTC ATG ATG GAC ACC |
FragMGL-R | CGA GGA TGT CGG CGA TGT CTT |
M13F | GTA AAA CGA CGG CCA G |
M13R | CAG GAA ACA GCT ATG AC |
The annealing temperatures were 49 and 50°C, except for primers M13F and M13R, where they were both 50°C.