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. 2004 Dec;70(12):7348–7354. doi: 10.1128/AEM.70.12.7348-7354.2004

TABLE 1.

Oligonucleotide primers used in this study for PCR amplificationa

Primer Sequence (5′-3′)
MGL−148 TTT TCC CCA CGC CCC GTG
MGL+148 GCA TGA CGA AAA GTG ACA
MGL-F ATG AGC ATC ACC CAG AAC
MGL-R TCA TAC CGT TGC TAC AGG
FragMGL-F TCG ACC TTC ATG ATG GAC ACC
FragMGL-R CGA GGA TGT CGG CGA TGT CTT
M13F GTA AAA CGA CGG CCA G
M13R CAG GAA ACA GCT ATG AC
a

The annealing temperatures were 49 and 50°C, except for primers M13F and M13R, where they were both 50°C.