TABLE 3.
Amino acid comparison of MGLs, CGSs, and CGLs by sequence alignmenta
| Gene (accession no.) | Residue at position no.b
|
Sequence of PLP-binding site (positions 194-206) | Residue at position 335 | Active site residues of MGL_Pp at positions 105, 177, 202, 368 | |||
|---|---|---|---|---|---|---|---|
| 41 | 81 | 107 | 110 | ||||
| MGL_Tv1 (CAA04124) | F | M | C | A | DVVVHSTKYING | V | Y, D, K, R |
| MGL_Tv2 (CAA04125) | F | M | C | A | DIVVHSAKYING | V | Y, D, K, R |
| MGL_Fn (AAL95612) | F | M | C | A | DIVVHSATKYLNG | V | Y, D, K, R |
| MGL_Pp1 (BAA20553) | F | M | C | A | DLVVHSATKYLSG | V | Y, D, K, R |
| MGL_Cc (NP_421962) | F | M | C | A | DLVVHSATKYLSG | V | Y, D, K, R |
| MGL_Oi (NP693968) | F | M | C | G | DFVIHSATKYISG | V | Y, D, K, R |
| MGL_Bc (NP834360) | F | M | C | G | DAVVHSATKYIGG | V | Y, D, K, R |
| MGL_Bh (BAB04518) | F | M | C | G | DFVVHSATKYIGG | V | Y, D, K, R |
| MGL_Ma (AAM05915) | F | M | C | S | DISLSSCTKYIGG | V | Y, D, K, R |
| MGL_Mm (NP635109) | F | M | C | S | DISLSSCTKYIGG | V | Y, D, K, R |
| MGL_Mb (ZP00075765) | F | M | C | S | DLSLSSCTKYIGG | Y, D, K, R | |
| MGL-B.linensc | P | T | G | R | DVVIHSTTKFING | E | Y, D, K, R |
| CGS_Mt (NP215595) | V | M | G | R | DVVLHSTTKYIGG | E | Y, D, K, R |
| CGS_Ml (NP302550) | V | M | G | R | DVVLHSTTKYIGG | E | Y, D, K, R |
| CGS_Bl (NP696324) | V | L | G | R | DVVVYSTTKYIGG | E | Y, D, K, R |
| CGS_Cg (NP601644) | P | M | G | R | HAVLHSTTKYIGG | E | Y, D, K, R |
| CGS_Cp (BAB79882) | F | L | G | R | DIVIHSATKYLGG | E | Y, D, K, R |
| CGS_Hp (P56069) | I | L | G | R | DIVAHSGTKYLGG | E | Y, D, K, R |
| CGS_Sa (BAB41649) | I | V | G | R | DIVLHSATKYLGG | E | Y, D, K, R |
| CGL_Hs (AAB24700) | A | L | G | R | DISMYSATKYMNG | E | Y, D, K, R |
| CGL_Rn (AAK52091) | A | L | G | R | DISMYSATKYMNG | E | Y, D, K, R |
| CGL_Ce (NP_495449) | N | L | G | R | DVVVHSITKYING | E | Y, D, K, R |
Multiple-sequence alignment was performed with Clustal W, version 1.8 (40). Names of organisms: Tv, Trichomonas vaginalis; Fn, Fusobacterium nucleatum; Pp, Pseudomonas putida; Cc, Caulobacter crescentus; Oi, Oceanobacillus iheyensis; Bc, Bacillus cereus; Bh, Bacillus halodurans; Ma, Methanosarcina acetivorans; Mm, Methanosarcina mazei; Mb, Methanosarcina barkeri; Mt, Mycobacterium tuberculosis; Ml, Mycobacterium leprae; Bl, Bifidobacterium longum; Cg, Corynebacterium glutamicum; Cp, Clostridium perfringens; Hp, Helicobacter pylori; Sa, Staphylococcus aureus; Hs, Homo sapiens; Rn, Rattus norvegius; Ce, Caenorhabditis elegans.
Position numbers correspond to MGL of B. linens.
The row corresponding to MGL of B. linens is in boldface type.