TABLE 3.
Total lipid content and fatty acid composition of recombinant yeast strainsa
| S. cerevisiae strain | Total lipids (nmol of acyl groups/mg of CDW) | Acyl composition (mol %)
|
||||
|---|---|---|---|---|---|---|
| C14:0 | C16:0 | C16:1 | C18:0 | C18:1 | ||
| G175(pESC-URA) | 194.9 | TR | 12.3 | 38.6 | 7.3 | 41.8 |
| H1246(pESC-URA) | 108.2 | TR | 5.3 | 38.0 | 8.8 | 47.9 |
| H1246(pESC-URA::atfA) | 138.5 | TR | 11.0 | 42.2 | 12.8 | 34.0 |
a
Cells were cultivated for 24 h at 28°C in synthetic minimal dropout medium without uracil and with 2% (wt/vol) galactose. Lyophilized cells were analyzed by GC. TR, trace (<1 mol%); CDW, cell dry weight.