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. 2017 Jan 19;16(5):428–435. doi: 10.1080/15384101.2017.1281476

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Figure 3. — Cell cycle-dependent changes in phosphorylation of Cdc25 are dependent upon Cdk1 consensus sites. (A) Cdc25 was analyzed by western blot in asynchronous wild type, cdc25-13A and cdc25Δ cdc2-3w cells. (B) Cdc25, Cdc13, and Cdk1 inhibitory phosphorylation were analyzed by western blot in cdc25–13A cells synchronized by centrifugal elutriation. a-tubulin was used as a loading control. (C) A fluorescence microscopy assay using DAPI and calcofluor staining was used to determine the percentage of binucleated cells and cells undergoing septation.