Figure 4.

Quiescent/dormant cancer cells induce nascent tumor vessels after chemotherapy. Experimental setup: FUCCI-expressing MKN45 cells (1×10⁷ cells/mouse) were injected subcutaneously into the left flanks of nestin-GFP transgenic nude mice. When the tumors reached approximately 6 mm in diameter (tumor volume, 80–100 mm³), mice were intraperitoneally injected with CDDP (4 mg/kg), PAX (5 mg/kg) or DOX (6 mg/kg) for 3 cycles every 3 d. Representative images of cross-sections of FUCCI-expressing MKN45 subcutaneous tumor of control (A), treated with CDDP (B), PTX (C), or DOX (D) 7 d and 21 d after last treatment. Low-magnification image (left). High-magnification image (right). Superficial area image (upper right). Deep area image (lower right). The cells in G₀/G₁, S, or G₂/M phases appear red, yellow, or green, respectively. (D) Histograms show the number of tumor vessels in the surface area or the center area in tumor of control, CDDP, PAX, or DOX 7 d after last treatment. (E) Bar graphs show the number of tumor vessels at the surface and deep area of control, CDDP-, PAX-, or DOX-treated tumors 21 d after last treatment. (F) Shows length of tumor vessels of control, CDDP-, PAX-, or DOX-treated tumors 21 d after last treatment. Data are shown as means ± SD (n = 5). Scale bars, 500 μm.

Figure 4.

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