Figure 3.

TSPO increased mitochondrial ATP production. (A) Averaged time-course of ATP production from permeabilised Jurkat cells was measured with luciferin-luciferase and a microplate reader. ATP production after injection of 2 mM ADP was markedly increased in all 3 cell types (wild-type, empty plasmid and TSPO-Jurkat cells). ATP production in TSPO-Jurkat cells increased at a greater rate than that in wild-type and empty plasmid Jurkat cells. (B) Accumulated ATP following injection of ADP in TSPO-Jurkat cells, calculated by subtracting the baseline (before ADP injection), was significantly greater than that observed in wild-type and empty plasmid Jurkat cells.