Table 4. Number of 16S rRNA gene sequencesa and taxa obtained from the digesta-adherent microbiome of all animals and sampling timesb.
Parameters | Total | Average per animal | SD | Min | Max |
---|---|---|---|---|---|
Trimmed readsc | 327300 | 15586 | 1324.4 | 12299 | 17341 |
Quality readsd | 110585 | 5266 | 360.4 | 4607 | 5986 |
Number of OTUse | 1539 | 821 | 70.3 | 653 | 926 |
Bacterial taxaf | 74 | 57 | 3.5 | 51 | 62 |
aAverage length of sequence (nt) = 519 and covers the V1-V3 region.
bNumber of barcoded samples = 21.
cSequence reads after quality trimming in QIIME.
dSequence reads after clustering with CD-HIT-OTU.
eNumber of OTUs clustered at 96% similarity.
fNumber of taxonomic groups, identified to the genus level if possible. The higher level taxa may contain several genera.