Table 1. Top metabolic pathways impacted by englerin A in renal cancer cells.
| Pathway Name (24 h) | Measured Metabolites in the Pathway (N) | Expected Pathway Proportion (P = N/599) | Expected Hits in Sample of 37 (P * 37) | Observed Hits in the Top 37 Metabolites | Fold Enrichment (Obs/Exp) | Impact (Sum VIP Score) | Fraction of Impact (VIP Score) Explained (% of 81.7) | Increased | Decreased |
| Sphingolipid Metabolism | 74 | 0.123 | 4.6 | 17 | 3.7 | 34.9 | 43% | 4 | 13 |
| Phospholipid Metabolism | 110 | 0.184 | 6.8 | 11 | 1.6 | 18.9 | 23% | 0 | 11 |
| Ganglioside Metabolism | 12 | 0.020 | 0.7 | 4 | 5.4 | 18.0 | 22% | 4 | 0 |
| Cardiolipin Metabolism | 12 | 0.020 | 0.7 | 1 | 1.3 | 2.6 | 3% | 0 | 1 |
| Pyrimidine Metabolism | 26 | 0.043 | 1.6 | 1 | 0.6 | 2.3 | 3% | 1 | 0 |
| Purine Metabolism | 34 | 0.057 | 2.1 | 1 | 0.5 | 1.8 | 2% | 1 | 0 |
| Cholesterol, Cortisol, Non-Gonadal Steroid Metabolism | 21 | 0.035 | 1.3 | 1 | 0.8 | 1.7 | 2% | 1 | 0 |
| Phosphate and Pyrophosphate Metabolism | 1 | 0.002 | 0.1 | 1 | 16.2 | 1.6 | 2% | 0 | 1 |
| Pathway Name (48 h) | Measured Metabolites in the Pathway (N) | Expected Pathway Proportion (P = N/599) | Expected Hits in Sample of 34 (P * 34) | Observed Hits in the Top 34 Metabolites | Fold Enrichment (Obs/Exp) | Impact (Sum VIP Score) | Fraction of Impact (VIP Score) Explained (% of 82.7) | Increased | Decreased |
| Fatty Acid Oxidation and Synthesis | 47 | 0.078 | 2.7 | 14 | 5.2 | 38.7 | 47% | 14 | 0 |
| Phospholipid Metabolism | 110 | 0.184 | 6.2 | 5 | 0.8 | 10.8 | 13% | 5 | 0 |
| Pyrimidine Metabolism | 26 | 0.043 | 1.5 | 5 | 3.4 | 10.5 | 13% | 5 | 0 |
| Ganglioside Metabolism | 12 | 0.020 | 0.7 | 3 | 4.4 | 7.3 | 9% | 3 | 0 |
| SAM, SAH, Methionine, Cysteine, Glutathione Metabolism | 21 | 0.035 | 1.2 | 2 | 1.7 | 4.5 | 5% | 1 | 1 |
| Purine Metabolism | 34 | 0.057 | 1.9 | 2 | 1.0 | 4.3 | 5% | 2 | 0 |
| Eicosanoid and Resolvin Metabolism | 22 | 0.037 | 1.2 | 1 | 0.8 | 2.7 | 3% | 1 | 0 |
Pathways impacted by Englerin A were determined using MetaboAnalyst 3.0 software after measuring metabolites from A498 cells treated with 0.1% DMSO or englerin A at 100 nM for 24 h and 48 h.