FIG. 1.

T-RFLP profiles in bacterial community DNA obtained from soil incubated in the absence or the presence of 50, 500, 2,000, or 5,000 ppm of 2,4,6-TCP. T0, nonincubated soil; T30, soil incubated for 30 days. The soil microbial community DNA was amplified using primer pairs 8F and 1392R and digested with the enzyme MspI. Fragment sizes (base pairs) that can be putatively assigned to bacterial genera with reported catabolic ability towards 2,4,6-TCP were the following: 141 to 143 (Burkholderia), 150 (Sphingomonas), 152 (Rhodococcus), 159 to 161 (Streptomyces), 159 (Nocardia), 163 (Arthrobacter), and 490 to 494 (Pseudomonas). The fragment size of 430 bp corresponding to Ralstonia was not found.