Fig 4. cpk31-1 and nip1;1–1 mutants contain less As (III) compared with the wild-type.

As (III) contents in shoots (A) and roots (B) of seedlings under As(III) conditions. Three-week-old seedlings of wild-type (Col-0), nip1;1–1 and cpk31-1 mutant grown under the hydroponic medium were transferred to the medium containing 10 μM As (III) for 12, 24, or 48 h. Samples were collected at indicated time points after treatment and As(III) contents were determined. Summarized As(III) content in (A) and in (B) was respectively deduced from results of 5 seedlings/condition of two or four replicate experiments among three independent experiments. Data are mean ± SD, and asterisks indicate statistically significant difference between Col-0 and nip1;1 or cpk31 mutant plants (*P< 0.05, Student’s t test).