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. 2017 Mar 15;12(3):e0173681. doi: 10.1371/journal.pone.0173681

Fig 6. Expression of CPK31 and NIP1;1 is sensitive to As(III).

Fig 6

The levels of expression of NIP1;1(A) and CPK31(B) in leaves and roots of the seedlings. 3-week-old plants grown at normal hydroponic medium were transferred to the medium with 10 μM As (III), and the roots and shoots of plants incubated for 0, 12, 24, 48, 72, or 144 h were gathered separately for mRNA measurement. After normalized to ACTIN2, the relative expression of NIP1;1 or CPK31 was calculated as the ratio of the normalized expression of NIP1;1 or CPK31 in shoot or root of seedlings treated with As(III) to that in shoot before treated with As(III). Data are mean ± SD of four replicate experiments with n = 3 for each experiment.