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. 2017 Mar 15;12(3):e0173681. doi: 10.1371/journal.pone.0173681

Fig 7. Growth phenotype of cpk31 nip1;1 double mutants under variable As(III) conditions.

Fig 7

(A) qRT-PCR analysis of CPK31, NIP1;1 and ACTIN2 gene expression from the wild-type (Col-0) and cpk31 nip1;1 double mutants (DM). Expression of ACTIN2 was analyzed as a quantitative control. (B) Growth phenotype of 10-day-old wild-type (Col-0), cpk31 nip1;1 double mutants (DM), nip1;1–1, and cpk31-1 mutants grown in half-strength MS agar medium containing 0 (a), 5 (b), 10 (c), 20 (d), or 40μM As(III) (e). The seeds of wild-type (Col-0), cpk31 nip1;1 double mutants (DM), nip1;1–1, and cpk31-1 mutants were firstly planted at the normal half-strength MS agar medium without As(III) for 4 days, and were then transferred for the medium containing various As(III) contents. Length of primary roots (C) and whole-plant biomass (D) of wild-type (Col-0), cpk31 nip1;1 double mutants (DM), nip1;1–1, and cpk31-1 mutants seedlings grown for 10 days under 0, 5, 10, 20, or 40 μM As(III). Data are mean ± SD of four replicate experiments. Asterisks indicate statistically significant difference between Col-0 and mutant plants exposed various contents of As (III) (*P< 0.05, Student’s t test).