Fig 4. Opn is dispensable for germinal center generation and IgA-producing B cells in PPs.

Eight-week-old female Opn KO and WT mice were cohoused for 3 weeks as described for Fig 3. Cells obtained from PPs were analyzed for detection of (a) GC B cell (GL7⁺CD95⁺ B220⁺ cells) and (b) IgA-producing B cells. Graphs show frequency and numbers of GC and IgA⁺ cells gated on B220⁺ cells. (c) Analysis of IgA-coating bacteria in the feces. Bacteria isolated from feces were stained with IgA and PI. PI⁺ events were considered to be bacteria. Graph shows the frequency of IgA⁺ cells with gating on PI⁺ cells. Bars indicate mean ±S.E.M. (n = 5; per group). Data are representative of four independent experiments.