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. 2017 Mar 15;12(3):e0173919. doi: 10.1371/journal.pone.0173919

Fig 2. Cleavage patterns of different staphylococcal endopeptidases using FRET-labeled and native peptides.

Fig 2

Peptidoglycan-like peptides (A) were incubated overnight with buffer only (B, E), lysostaphin (C, F), or PlySs2 (D, G). Both FRET-labeled (B-D), and native peptides (E-G) were used. Generated fragments were detected using LC-MS and analyzed using Skyline. Panels B through G: Measured signals are shown on the y-axis. N-terminal fragments are shown as positive values (black bars) while fragments corresponding to the C-termini are shown with light grey bars. Because the C-terminal signals were found to be very low, the values were multiplied by 50 for visualization purposes. Standard deviations for the replicated measurements are shown. The x-axis shows the origin of the signal with respect to the peptidoglycan-like peptide.