TABLE 2.
Primers used for MLST of O. oeni strains
| Protein | Accession no.a | Gene | Primers | Sequence 5′→3′ | 5′ start positiona | PCR product length (bp) | Accession no. |
|---|---|---|---|---|---|---|---|
| B subunit of DNA gyrase | ZP_00069208 | gyrB | GYRB1 | TGGGCTTCATGGTGTTGGC | 375 | 947 | AJ618989-AJ618996 |
| GYRB2 | CCCTCGACGATAAACAATTC | 1322 | |||||
| d-Alanine-d-alanine ligase | ZP_00069716 | ddl | DDL1 | CGATGTTAGCAAGCGTTCG | 54 | 911 | AJ618997-AJ619006 |
| DDL2 | TTCGTATTTCCCGGTAGTG | 965 | |||||
| Phosphoglucomutase | ZP_00070472 | pgm | PGM1 | CATCCGACTCCGGAATTGAC | 370 | 826 | AJ619007-AJ619018 |
| PGM2 | CCGTAGGATTCTTCAAAACC | 1196 | |||||
| Transketolase | ZP_00069744 | recP | RECP1 | GGCGATGGGGACTTAATGG | 550 | 926 | AJ619666-AJ619673 |
| RECP2 | CCCGTCTTCGCCGACAGC | 1476 | |||||
| Malolactic enzyme | ZP_00069821 | mleA | OO1b | GTGCCGCTTTTTTGGATATTA | 332 | 430 | AJ619674-AJ619675 |
| OO2b | AGCAATTTTATCTTTATAGCT | 762 |
a
The protein accession number and the indication of the gene position refer to the uncompleted genome sequence of O. oeni MCW (formely, PSU-1) by the DOE Joint Genome Institute (http://genome.jgi-psf.org/draft_microbes/oenoe/oenoe.home.html).
b
The mleA primers have been described by Divol et al. (9).