Figure 5. Benztropine mesylate partially impairs mammosphere formation of breast CSCs through acetylcholine receptors, dopamine receptors/transporters and histamine receptors.

Representative images A. and quantification of mammosphere formation efficiency B. of 1,000 4T1-luc2 cells co-treated with benztropine mesylate (5 μM) and dopamine (10 μM), histamine (5 μM) or carbachol (5 μM) alone or with an agonist combination (dopamine + histamine + carbachol) for 6 days. Quantification of mammosphere numbers from 1,000 4T1-luc2 cells treated with either the muscarinic receptor antagonist atropine D. or the dopamine receptor antagonist haloperidol E. for 6 days. Data are expressed as mean±SD (n=6). **p<0.01, ***p<0.001 compared with DMSO control (One-way ANOVA).