Figure 3. Expression of anti-apoptotic Bcl-2 family proteins in PEO1 and PEO4 cells in response to treatment with cisplatin, TCN and NU7441.

Cisplatin-sensitive PEO1 and cisplatin-resistant PEO4 cells were treated as indicated. Cell lysates were prepared and were separated by 12% SDS-PAGE gel as described in Materials and Methods. (A) Blots were probed with anti-apoptotic pBcl-2 (Ser70), total Bcl-2, Mcl-1, Bcl-XL, and XIAP and all membranes were incubated with β-tubulin, which was used as a loading control. (B) Densitometry values for protein expression in PEO4 cells analyzed by Image J software and normalized to their corresponding β-tubulin densities.