Table 1. Validated antibodies used for Western blotting (WB) experiments.
| Antibody | Dilutions | Solvent | Supplier | Molecular Weight |
|---|---|---|---|---|
| pAKT (Ser473) (Rabbit) | 1:1000 | 5% BSA in TBST | Cell Signaling | 60 kDa |
| pSrc (Tyr416) (Rabbit) | 1:1000 | 5% BSA in TBST | Cell Signaling | 60 kDa |
| Cyclin B1 (Mouse mAb) | 1:250 | 5% milk in TBST | Santa Cruz | 60 kDa |
| Pro-apoptotic Bcl-2 family proteins and Caspases | ||||
| Bax (Rabbit mAb) | 1:1000 | 5% milk in TBST | Cell Signaling | 20 kDa |
| Bak (Rabbit mAb) | 1:1000 | 5% milk in TBST | Cell Signaling | 25 kDa |
| Bim (Rabbit mAb) | 1:1000 | 5% milk in TBST | Cell Signaling | 12, 15, 23 kDa |
| BID (Human specific) | 1:1000 | 5% milk in TBST | Cell Signaling | 15,22 kDa |
| Puma (Rabbit Ab) | 1:1000 | 5% milk in TBST | Cell Signaling | 23 kDa |
| Bik (Rabbit Ab) | 1:1000 | 5% milk in TBST | Cell Signaling | 20 kDa |
| Bad (Rabbit mAb) | 1:1000 | 5% milk in TBST | Cell Signaling | 23 kDa |
| P-Bad (Ser 136) (Rabbit) | 1:1000 | 5% BSA in TBST | Cell Signaling | 23 kDa |
| Caspase 8 (Mouse mAb) | 1:1000 | 5% milk in TBST | Cell Signaling | 18, 43, 57 kDa |
| Caspase 9 (Rabbit) | 1:1000 | 5% milk in TBST | Cell Signaling | 17, 35, 37, 47 kDa |
| Anti-apoptotic Bcl-2 family proteins | ||||
| pBcl-2 (Ser70) (Rabbit) | 1:1000 | 5% BSA in TBST | Cell Signaling | 28 kDa |
| pBcl-2 (Thr56) (Rabbit) | 1:1000 | 5% BSA in TBST | Cell Signaling | 28 kDa |
| Bcl-2 | 1:1000 | 5% milk in TBST | Cell Signaling | 26 kDa |
| XIAP | 1:1000 | 5% milk in TBST | Cell Signaling | 53 kDa |
| Mcl-1 (Rabbit mAb) | 1:1000 | 5% milk in TBST | Cell Signaling | 40,35 kDa |
| Bcl-XL (Rabbit mAb) | 1:1000 | 5% milk in TBST | Cell Signaling | 30 kDa |
| β-tubulin | 1:5000 | 5% milk in TBST | Santa Cruz | 55 kDa |
| Anti-rabbit-HRP | 1:2000 | 5% milk in TBST | Dako | NA |
| Anti-mouse-HRP | 1:5000 | 5% milk in TBST | Dako | NA |
All antibodies are diluted as specified in the table. p-, phosphor-; mAb, monoclonal antibody; pAb, polyclonal antibody; NA, not applicable.