TABLE 1.
Strain or plasmid | Description | Source or reference |
---|---|---|
E. coli strains | ||
DH5α | F− φ80lacZΔM15Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17 (rK− mK+) gal-phoA supE44 λ−thi-1 gyrA96 relA1 | www.invitrogen.com |
DY378 | W3110 λcI857 Δ(cro-bioA) | 9 |
Plasmids | ||
pACYC184 | p15A origin of replication (Tetr Chlr) | 1 |
pFull-cat | pACYC184 derived; PPrcat (Tetr Chlr) | This study |
pTrun-cat | pACYC184 derived; PPrtruncatedcat (Tetr Chllow) | This study |
pKD46 | ParaB γ β exo (Ampr) | 3 |