TABLE 2.

List of oligonucleotides used in this study

Oligonucleotide	Sequencea
Tru-cat-for	5′-tatagaattcttacctctggcggtgataatggttgcatgtactaaggaggttgtatggagaaaaaaatcactgg-3′
Full-cat-for	5′-tatagaattcgtgttgactattttacctctggcggtgataatggttgcatgtactaaggaggttgtatggagaaaaaatcaactgg-3′
NcoI-cat-rev	5′-cttaccatggttacgccccgccctgcc-3′
Km-for-full	5′-ctccatacaacctccttagtacatgcaaccattatcaccgccagaggtaaAATAGTCAACACtgtaggctggagctgctt-3′
Km-for-rrnA1	5′-tccagtgatttttttctccatacaacctccttagtacatgcaaccattatAGGGAGTTATTCCGGCCTGACAAGTgtaggctggagctgctt-3′
Km-rev-790	5′-ttgccgcggccctctcacttccctgttaagtatcttcctggcatcttccaatgggaattagccatggtc-3′
lacZ-for-full	5′-ctccatacaacctccttagtacatgcaaccattatcaccgccagaggtaaAATAGTCAACACcgttggccgattcatta-3′
lacZ-rev	5′-ttgccgcggccctctcacttccctgttaagtatcttcctggcatcttccagagcttgacggggaaag-3′
Seq1-pos670-rev	5′-CATATTCTGCTGACGCA-3′
seq2-pos900-rev	5′-gatttgagcgtcagatttcg-3′
seq3-pos161-for	5′-tctttacgatgccattg-3′
T7	5′-gtaatacgactcactatagggc-3′

^aItalics indicate flanking homology to plasmid. Boldface indicates completion of either λP_R or rrnA1 promoter.