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A. A panel of six NB cell lines were seeded in six-well plates with indicated concentrations of afatinib in soft agar, and grown for 2 to 3 weeks, followed by staining with crystal violet for 4 hrs and the photos were taken. B. Colonies were counted and colony numbers were presented as mean ± SD. P <0.01 (**), or P <0.001 (***) (Student's t-test) were indicated.
