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A-F. IMR-32, NGP, NB-19, SK-N-AS and SH-SY5Y and LA-N-6 cells were treated with afatinib (10 μM or 20 μM) for various time points (0-16 hrs). At the end of treatment, cells were harvested and cell lysates were subjected to SDS-PAGE, and then immunoblotted with the indicated antibodies. β-Actin was used as a loading control.
