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. 2016 Nov 26;8(1):1555–1568. doi: 10.18632/oncotarget.13657

Figure 6. Afatinib enhances doxorubicin-induced cytotoxicity in NB cells.

Figure 6

A. Six cell lines were seeded in 96-well plates and were incubated with doxorubicin at the indicated concentrations with or without afatinib (2 μM) for 48 hrs. Cell viability was then measured by CCK-8 assay. Data were represented as mean ± SD. P <0.05 (*), P <0.01 (**) or P <0.001 (***) (Student's t-test) are indicated. B. IMR-32, NGP, NB-19, SK-N-AS, SH-SY5Y, and LA-N-6 cells were treated with either doxorubicin (2 μM) alone, afatinib (2 μM) alone, or their combinations for 16 hrs. Then whole cell lysates were then subjected to SDS-PAGE and immunoblotted with the PARP and Caspase 3 antibodies. β-Actin was used as a loading control in all samples.