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. 2016 Dec 1;8(1):1641–1654. doi: 10.18632/oncotarget.13767

Figure 2. CD82 suppresses chemotactic migration and invasiveness of prostate cancer cells.

Figure 2

A. Chemotactic cell migration assay using Transwell-chamber inserts was performed as described in “Materials and Methods”. Results are the mean ± s.d. from three separate experiments performed in triplicate (*, **, and ‡, p < 0.03; †, p < 0.01 versus mock; Student's t-test). B. Cells were labeled with a fluorescent cell tracker, CMTMR (red), and seeded atop the chick chorioallantoic membrane (CAM) of 11-day-old chick embryos. After three days incubation, the embryos were frozen and cross-sectioned. CAM sections stained with DAPI (blue) were photographed under a fluorescent microscope. The CAM surface is marked with a dashed line. Cells showing both red and blue fluorescence beneath the CAM surface were counted and expressed as the mean ± s.d. of five sections in each of the four embryos.