Figure 3. CD82 inhibition of fibronectin-induced EMT is dependent on α₃β₁ and α₅β₁ integrins.

A. Stably CD82-transfected DU145 and antisense CD82 fragment-transfected LNCaP cell clones suspended in culture medium were pretreated with a mouse normal IgG₁ or integrin β₁-blocking IgG₁ for 2 hours and cultured on plates precoated with fibronectin (FN) for 8 hours. Cells in suspension (Susp), and cells grown onto the plates were examined for expression of E-cadherin and Snail using immunoblotting analysis. Bar graphs under the immunoblots indicate the relative band intensity of E-cadherin and Snail normalized to the loading control (actin). Values are the mean ± s.d. from three separate experiments performed in triplicate (*, **, †, and ‡, p < 0.03). ND, not detectable. B-D. Cells grown on FN were transfected with either scrambled (scrmb) siRNAs or integrin α₃ (B), α₅ (C), or α₆ (D) subunit-specific siRNAs and then examined for E-cadherin and Snail expression.