Skip to main content
. 2016 Dec 2;8(1):1678–1687. doi: 10.18632/oncotarget.13779

Figure 2. IL-1β induces the transcription of miR-31 via p38 and JNK.

Figure 2

a. The pri-miR-31 levels were measured relative to GAPDH mRNA using quantitative reverse transcription-PCR (qRT-PCR). b-c. HUVEC endothelial cells were pre-treated with 5μM of p38 inhibitor SB203580 or 10μM of JNK inhibitor SP600125 for 1 hour before the addition of IL-1β (20ng/ml). The inhibitions were confirmed by Western blotting showing decreased of phospho-HSP27 (P~HSP27) and phospho-c-Jun (P~c-Jun), downstream of p38 and JNK, respectively. The endogenous HSP90 was used as loading control. Pri-miR-31 levels relative to GAPDH mRNA were determined by qRT-PCR. The Western blots are representative of four independent experiments. The error bars represent standard errors of four independent experiments and significance was analyzed using a Student's t-test (*p <0.05; **p<0.01).