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. 2016 Dec 10;8(1):1714–1724. doi: 10.18632/oncotarget.13852

Figure 5. NEDD9 regulated cancer stem cell phenotype through Smad7 in hepatocellular carcinoma cells.

Figure 5

Western blots were used to test A. expression of NEDD9 and Smad7 in a side population (SP) and main population (MP) isolated by fluorescence-activated cell sorting. B. Expression of NEDD9 and Smad7 in suspension (Sus) and attached (Adh) hepatocellular carcinoma (HCC) cells. C. Expression of Smad7 in NEDD9-overexpressing and NEDD9-knockdown HCC cells. D. Quantitative PCR was used to test mRNA of Smad7 in NEDD9-overexpressing and NEDD9-depleted HCC cells. E. Sphere-formation assays were used to test sphere-forming abilities of NEDD9-overexpressing and NEDD9-depleted HCC cells. Scale bar, 100 μm. * p <0.01 compared to control cells.